Overview

This trial is active, not recruiting.

Condition preimplantation diagnosis
Treatments vegetal pole blastomer embryo, animal pole blastomer embryo
Sponsor Ahmad Mustafa Mohamed Metwalley
Collaborator Ibn Sina Hospital
Start date January 2015
End date August 2016
Trial size 100 participants
Trial identifier NCT02902614, Embryo Biopsy

Summary

Selection of side of embryo biopsy that will not interfering with implantation power of developed blastocyst embryo during biopsy procedure arranged for PGT.

United States No locations recruiting
Other Countries No locations recruiting

Study Design

Allocation non-randomized
Endpoint classification safety/efficacy study
Intervention model crossover assignment
Masking open label
Primary purpose supportive care
Arm
(Experimental)
for day 3 embryo biopsy / cleavage stage: Blastomere collection from opposite side to the 2nd polar body or from vegetal pole.
vegetal pole blastomer embryo Biopsy embryo from sides considered far from ICM location.
for day 3 embryo biopsy / cleavage stage
animal pole blastomer embryo Random biopsy without considering specific location
for day 3 embryo biopsy / cleavage stage
(Experimental)
for day 3 embryo biopsy / cleavage stage: Blastomere collection from and side around the 2nd polar body and not the vegetal pole.
vegetal pole blastomer embryo Biopsy embryo from sides considered far from ICM location.
for day 3 embryo biopsy / cleavage stage
animal pole blastomer embryo Random biopsy without considering specific location
for day 3 embryo biopsy / cleavage stage

Primary Outcomes

Measure
blastocyst location of ICM at day 5
time frame: 2 months

Secondary Outcomes

Measure
Implantation power
time frame: 2 months

Eligibility Criteria

Male or female participants of any age.

Inclusion Criteria: - grade 1 or 2 embryos at eight cell stage or 8-1/ 8-2 embryo quality. Exclusion Criteria: - grade 3 embryo at day 3, or 8-3 embryos.

Additional Information

Official title Effect of Embryo Biopsy Side on Post Biopsy Blastocyst Structurs and Implantation
Principal investigator Ahmad M Metwalley
Description During process of embryo biopsy, it was indicated to avoid contacting cells closer to inner cell mass as it can decrease the quality of embryo and its structural composition. However, recommendation for collection from the trophectoderm (TE) side is indicated. Absence of differentiation during cleavage-stage or morula stage at day 3 and day 4 respectively, will present the needful to focus a specific location for embryo biopsy during pre-blastocyst stages for embryo going to do genetic testing before implantation or embryo transfer. In articulation around the second polar body or the animal pole of embryo, collection of the cell from the vegetal pole in crospondence to avoide collection form the animal pole is to be compare the results of implantation for biopsy done using the animal pole side or the random non organized biosy procedure.
Trial information was received from ClinicalTrials.gov and was last updated in September 2016.
Information provided to ClinicalTrials.gov by Al Baraka Fertility Hospital.