This trial is active, not recruiting.

Conditions increased metabolic requirement, healthy
Treatment amino acid intake
Phase phase 0
Sponsor University of Toronto
Collaborator Ajinomoto Co., Inc.
Start date April 2015
End date June 2016
Trial size 7 participants
Trial identifier NCT02478814, IAAO-YM


Protein requirements in active individuals have been suggested to be greater than the current recommended dietary allowance (RDA).

Nutritional requirements for dietary amino acids in adults have traditionally been determined utilizing the nitrogen balance technique, which is prone to underestimating protein requirements. As a result, there is a need to re-evaluate recommendations in order to characterize how dietary amino acid needs may be modulated by physical activity.

Recent studies using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 50% higher than the RDA based on nitrogen balance data.

The purpose of this study is to measure the protein requirement in active, young adult males using the IAAO technique in the presence of a variable intensity exercise stimulus.

United States No locations recruiting
Other countries No locations recruiting

Study Design

Intervention model single group assignment
Masking open label
Primary purpose basic science
Subjects will receive different levels of amino acid intakes varying from 0.2 to 2.6 g/kg/day.
amino acid intake
Amino acid intakes will be varied at 0.2-2.6 g/kg/day

Primary Outcomes

13CO2 (carbon dioxide) excretion (μmol/kg/h)
time frame: 4 hours / study day

Secondary Outcomes

13C-Phenylalanine Oxidation (μmol/kg/h)
time frame: 4 hours / study day

Eligibility Criteria

Male participants from 18 years up to 35 years old.

Inclusion Criteria: - Healthy, active, young adult males who regularly participate in moderate-vigorous activity 5x per week, for a minimum of 1 hour - A minimum score of 10 on the Beep Test (a measure of aerobic fitness) Exclusion Criteria: - Inability to meet health and physical activity guidelines according to the physical activity readiness questionnaire (PAR-Q+) - Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption) - Regular tobacco use - Illicit drug use

Additional Information

Official title Evaluation of Protein Requirements in Active, Young Adult Males
Trial information was received from ClinicalTrials.gov and was last updated in August 2016.
Information provided to ClinicalTrials.gov by University of Toronto.