Overview

This trial is active, not recruiting.

Conditions aging, obesity
Treatment overfeeding
Sponsor University of Colorado, Denver
Collaborator Colorado State University
Start date June 2015
End date June 2016
Trial size 30 participants
Trial identifier NCT02456870, 14-2411

Summary

The purpose of this pilot study is to determine whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. The investigators hypothesize that the adipose tissue response to overfeeding may be weakened in older when compared to younger men, thereby leading to worsened ectopic storage depots for older men.

United States No locations recruiting
Other Countries No locations recruiting

Study Design

Allocation non-randomized
Intervention model single group assignment
Masking open label
Primary purpose basic science
Arm
(Other)
Middle-aged overweight and obese men (age 25-40yr)
overfeeding
Consumption of 40% excess calories for 7 consecutive days
(Other)
Older overweight and obese men (age 55-75yr)
overfeeding
Consumption of 40% excess calories for 7 consecutive days

Primary Outcomes

Measure
Change in adipose-derived stem cell (ASC) differentiation (Delta%oil red O+)
time frame: 1 wk of overfeeding

Secondary Outcomes

Measure
Change in ASC proliferation (Delta rate of cell growth)
time frame: 1 wk of overfeeding
Change in expression of C/EBPalpha, C/EBPbeta, PPARgamma2, SREBP1c, SREBf1a, and Wnt10b (fold-changes in mRNA expression)
time frame: 1 wk overfeeding
Change in intramyocellular (IMCL) di-saturated diacylglycerol (DAG) localization (Delta %membrane:cytosol)
time frame: 1 week overfeeding

Eligibility Criteria

Male participants from 25 years up to 75 years old.

Inclusion Criteria: - Men - Aged 25-40y OR 55-75y - BMI 28-35 kg/m2 - Weight stable (±2kg over past 3 mo) - Sedentary to moderately active (<3 d/wk of exercise lasting >20min) - Non-smokers (never used or quit >7yrs prior) Exclusion Criteria: - Women - Currently using hormones, glucose or lipid lowering medication - Engaged in exercise or diet program - Currently gaining or losing weight or using weight loss drugs - Diabetes mellitus (diagnosis, treatment, FBG ≥126 mg/dL) - Uncontrolled metabolic disorders (e.g., thyroid)

Additional Information

Official title Aging Subcutaneous Adipose Tissue Response to Overfeeding
Principal investigator Rachael E Van Pelt, PhD
Description This pilot study will begin to address whether adipose tissue biology changes with advancing age. Specifically, we will test whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. To test this possibility we will recruit overweight and mildly obese older (age 55-75y) and younger (age 25-40y) men and collect adipose tissue biopsies before and after 7 days of overfeeding. We will test how well the cells in adipose tissue expand in response to overfeeding and whether this differs between younger and older men.
Trial information was received from ClinicalTrials.gov and was last updated in July 2016.
Information provided to ClinicalTrials.gov by University of Colorado, Denver.