Overview

This trial is active, not recruiting.

Conditions sleep, sleep deprivation
Treatments sleep deprivation, normal sleep
Sponsor Federal University of São Paulo
Collaborator Fundação de Amparo à Pesquisa do Estado de São Paulo
Start date July 2013
End date December 2013
Trial size 10 participants
Trial identifier NCT02082600, 2010070050

Summary

The aims of this study are examine the effects of sleep deprivation in muscle recovery after a maximum eccentric resistance exercise session performed on an isokinetic dynamometer (24 series of 10 repetitions). The sample will consist of 10 men, sedentary, clinically healthy, aged between 20 and 31 years old. Two experimental groups will be developed: EXE-SLEEP, in which subjects will perform the exercise protocol (~18:00-19:00) and will be subject to normal period of sleep for 3 nights; EXE-TOTAL, in which subjects will perform the same exercise protocol and will be sleep deprived for 60 hours, followed by one night of sleep rebound.

United States No locations recruiting
Other Countries No locations recruiting

Study Design

Allocation randomized
Intervention model crossover assignment
Masking open label
Primary purpose basic science
Arm
(Experimental)
Exercise induced-muscle damage protocol followed by 60h of sleep deprivation (two nights) and one night of normal sleep (rebound).
sleep deprivation Sleep and muscle recovery
This group will perform 24 sets of 10 maximum repetitions of eccentric contractions for quadriceps and than will keep awake for 60 hours (2 nights), followed by 1 night of sleep, all inside the lab. During whole protocol, it will be collected several blood samples for evaluation of hormones, cytokines and muscle damage parameters. It will be also evaluated isometric strength and pain.
(Experimental)
Exercise induced-muscle damage protocol followed by 3 nights of normal sleep
normal sleep
This group will perform 24 sets of 10 maximum repetitions of eccentric contractions for quadriceps and and will be able to sleep the 3 nights. During whole protocol, it will be collected several blood samples for evaluation of hormones, cytokines and muscle damage parameters. It will be also evaluated isometric strength and pain.

Primary Outcomes

Measure
Change from baseline in isometric strength
time frame: Before exercise, immediatly after, 12, 24, 36, 48, and 60 hours after exercise induced-muscle damage

Secondary Outcomes

Measure
Area Under Curve for serum total and free testosterone, IGF-1, and cortisol
time frame: Blood samples will be collected every 2 hours, for 24 hours.

Eligibility Criteria

Male participants from 20 years up to 31 years old.

Inclusion Criteria: - healthy subjects - sedentary - sleep duration between 6-8 hours peer night Exclusion Criteria: - smokers - use vitamin supplements, like C and E - drink two doses of alcohol/day or > 4 doses in one occasion - use of anti-inflammatory drugs - antilipidemic drugs - anemia - muscle or joint diseases - cardiovascular diseases - diabetes - renal diseases - sleep disturbances - breathing disorders

Additional Information

Official title Effects of Sleep Deprivation on Blood Hormones and Inflammatory Status After Exercise Induced-muscle Damage
Principal investigator Marco Tulio Mello, PhD
Description The blood parameters analyzed will be: - Creatine kinase and myoglobin: before, immediately after, 2, 4, 12, 24, 36, 48, and 60 hours after exercise protocol. - Total and free testosterone, IGF-1, and cortisol: before, immediately after, 2, 4, 12, 24, 36, 48, and 60 hours after exercise protocol. Moreover, from 19:00 of the second day to 19:00 of the third day it will be collected blood samples every 2 hours. - GH: during the second night, GH will be evaluated every 1 hour, from 23:00 to 07:00. - TNF-alfa, IL-6, IL-1 beta, IL-10, and IL-1ra: before, immediately after, and 2 hour after exercise, as well every 4 hours from 19:00 of the second day to 19:00 of the third day. It will be evaluated isometric strength every 12 hours, as well visual analog scale of pain.
Trial information was received from ClinicalTrials.gov and was last updated in March 2014.
Information provided to ClinicalTrials.gov by Federal University of São Paulo.