This trial is active, not recruiting.

Condition acute myeloid leukemia
Treatment allogenic nk cells infusion
Phase phase 1/phase 2
Sponsor Assistance Publique - Hôpitaux de Paris
Start date May 2011
End date December 2012
Trial size 11 participants
Trial identifier NCT01947322, P070505


Leukemia cells can be killed by natural killer (NK) from HLA-I mismatched donor. The proposed study plans to realize an adoptive anti-leukaemic immunotherapy by infusion of HLA-I mismatched NK cells to treat poor prognosis acute myeloid leukemia patients. NK cells will be selected from HLA mismatch familial donor peripheral mononuclear cells by purification protocol. Before NK-infusion, patients received immunosuppressive chemotherapy.

United States No locations recruiting
Other Countries No locations recruiting

Study Design

Endpoint classification safety/efficacy study
Intervention model single group assignment
Masking open label
Primary purpose treatment
allogenic nk cells infusion cellular therapy
HLA Haploidentical selected NK cell infusion (one injection of 1x107/kg CD3-CD56+ cells) after chemotherapy associating fludarabine, cytosine arabinoside and cyclophosphamide.

Primary Outcomes

duration of neutropenia inferior to 500 neutrophils /mm3
time frame: from the day of NK infusion (day 0) up to 35 days

Eligibility Criteria

Male or female participants from 18 years up to 65 years old.

1. Recipient selection 1. Patient eligibility - Poor prognosis de novo AML including : 1. Primary refractory disease (absence of complete remission (CR) after at least 2 different induction regimens) 2. Relapsed disease that did not reach CR after at least 1 salvage therapy 3. First untreated early relapse (less than one year of remission duration) in the absence of allogeneic HSCT project. - Age between 18 and 65 - No liver and renal dysfunctions contraindicating the administration of Fludarabine, Cyclophosphamide or Cytarabine. - Written informed consent 2. Patient exclusion criteria - Secondary AML. - Previous autologous or allogeneic transplantation. Since the main objective of the study concerns the hematological toxicity, we decided to exclude patients with secondary AML or who had been previously transplanted because of their expected higher hematological toxicity. - Patient with allogeneic transplant project - HIV positive serology 2. Donor eligibility - HLA haploidentical brother, sister, child (older than 18 years), father, sister, cousin, uncle, aunt. - Donor with KIR ligand mismatch in the GvL direction - Absence of contraindication for leukapheresis. - Negative HIV1-2, HTLV-1-2, HBV, and HCV serology. Negative viral genomic screening for HTLV1-2 and HCV - Written informed consent

Additional Information

Official title Haploidentical NK-cell Infusion in Bad Prognosis AML Patients: Evaluation of Feasibility and Antitumoral Effect
Principal investigator Nathalie Dhedin, MD
Description NK cell-mediated cytotoxity is regulated by signals provided by surface inhibitory and activating receptors. Target cells will be killed in the absence of interaction between NK inhibitory receptors and their ligands (HLA class I molecules) on the target cells. The proposed phase I/II study plans to realize an adoptive anti-leukaemic immunotherapy by infusion of haploidentical HLA-I mismatched NK cells to treat poor prognosis AML patients. Familial donors of NK cells will be selected according to their HLA typing in order to choose a donor with NK cells expressing at least one inhibitory receptor that can not recognize any HLA class I molecule on recipient cells. NK cells will be selected from donor peripheral mononuclear cells by a two step purification protocol (CD3 negative with subsequent CD56 positive selections). NK cells will be then activated ex vivo overnight in the presence of IL-2 before infusion. In vivo IL-2 injections will be performed for 14 days. Before NK-infusion, patients will be conditioned by a cytoreductive and immunosuppressive chemotherapy. An extensive biological study of NK cells will be performed in the recipient post-infusion, including chimerism analyses, phenotypic and functional tests in order to evaluate NK-cell expansion post-infusion and their capacity to mediate an antitumoral effect. Since donor NK cells have been selected for their potential graft versus host (GvH) and graft versus leukemia (GvL) reactivity, such approach might induce prolonged cytopenia due to a direct toxicity of NK cells against normal hematopoietic progenitors. The main goals of this study will be thus to evaluate (1) the hematological feasibility of allogeneic NK-cell infusion, (2) the expansion of the infused population, (3) an antitumoral effect mediated by this adoptive immunotherapy. This is an essential step before further development of such anti-tumoral immunotherapeutic approach, in leukemic patients but also in solid tumors that could be sensitive to an "NK-effect" (melanoma, kidney cancer). This project includes 4 clinical departments and several laboratories of cellular therapy and immunology that have got an expertise in the field of Acute Myeloid Leukemia (AML), cellular therapy and NK-cell.
Trial information was received from ClinicalTrials.gov and was last updated in September 2015.
Information provided to ClinicalTrials.gov by Assistance Publique - Hôpitaux de Paris.