Overview

This trial is active, not recruiting.

Condition old age (focus; not a recognized condition)
Treatments 15g eaa bolus, 4 x 3.75g mixed eaa pulses, 15g eaa bolus supplemented with 3g arginine
Sponsor University of Nottingham
Start date December 2011
End date January 2014
Trial size 40 participants
Trial identifier NCT01735539, UNottingham F/3/2009

Summary

The investigators plan to measure changes in muscle protein metabolism in response to feeding, comparing between a single large essential amino acid (EAA) feed (the normal building blocks of protein) and the provision of the same dose in 4 smaller feeds at 45min intervals. The investigators will perform this study in healthy older (65-75y) and younger (18-28) men. The investigators will also explore how feeding affects muscle blood flow as this is important in the delivery of the nutrients we eat to the muscle where they are used.

The investigators plan to supplement the feed with arginine, a safe and widely found non-essential amino acid, to explore if this can improve muscle blood flow.

United States No locations recruiting
Other Countries No locations recruiting

Study Design

Allocation non-randomized
Intervention model parallel assignment
Masking double blind (subject, investigator)
Primary purpose treatment
Arm
(Experimental)
15g EAA bolus
15g eaa bolus Mixed Essential Amino Acids
Oral; in aqueous solution
(Experimental)
15g EAA Bolus supplemented with 3g Arginine
15g eaa bolus supplemented with 3g arginine
Oral; in aqueous solution
(Experimental)
15g EAA bolus
15g eaa bolus Mixed Essential Amino Acids
Oral; in aqueous solution
(Experimental)
4 x 3.75g Mixed EAA Pulses
4 x 3.75g mixed eaa pulses
Oral; in aqueous solution
(Experimental)
4 x 3.75g Mixed EAA Pulses
4 x 3.75g mixed eaa pulses
Oral; in aqueous solution

Primary Outcomes

Measure
Muscle Protein Synthesis (FSR, fractional synthetic rate, %/hr)
time frame: Across 6hr study

Secondary Outcomes

Measure
Muscle Protein Breakdown (MPB)
time frame: Across 6hr study
Whole leg blood flow
time frame: Across a 6hr study
Muscle Microvascular Blood flow
time frame: Across a 6hr study

Eligibility Criteria

Male participants from 18 years up to 75 years old.

Inclusion Criteria: - age 65-75 or 18-28 Exclusion Criteria: - BMI >30 - Diabetes - Beta blocker or steroid use - Established cerebrovascular, peripheral vascular or ischaemic heart disease.

Additional Information

Official title Dysfunction of Nutritive Blood Flow as a Determinant of Anabolic Resistance in Older People; The Role of Essential Amino Acids in Modulating Muscle Protein Metabolism; Bolus vs. Pulse Feeding Strategies and the Ability of Arginine to Rejuvenate Microvascular Responsiveness.
Principal investigator Philip J Atherton, PhD
Description We will provide a 15g mixed essential amino acid feed or 4 x 3.75g. There is sexual dimorphism thus initially we will just recruit males. Powering calculations dictate n=8 in each group. We will use well established stable isotope tracer techniques (with 13C6 Phenylalanine) to measure muscle protein synthesis and breakdown by Gas Chromatography - combustion - isotope ratio mass spectroscopy. We will measure leg blood flow by phase shift Doppler and muscle microvascular flow by contrast enhanced ultrasound.
Trial information was received from ClinicalTrials.gov and was last updated in January 2014.
Information provided to ClinicalTrials.gov by University of Nottingham.