This trial is active, not recruiting.

Conditions methylene blue, laparoscopy, tubal patency
Treatments methylen blue &normal saline, methylene blue, metheylne blue with normal saline
Sponsor Pourmatroud, Elham, M.D.
Start date September 2012
End date February 2013
Trial size 30 participants
Trial identifier NCT01725477, Methylen blue, Methylen blue in laparoscopy, Methylen blue in laparoscopy


1. Methylene blue in laparoscopy use for tubal patency assessment

2. Methylene blue could have negative effect on sperm motility, oocyte granulosa cell and fertility.

3. tubal washing after Methylene blue administration could be effective in pregnancy rate after operation.

United States No locations recruiting
Other Countries No locations recruiting

Study Design

Allocation randomized
Endpoint classification efficacy study
Intervention model crossover assignment
Masking double blind (subject, outcomes assessor)
Primary purpose treatment
first arm:20 ml methylene blue washing with50 ml normal saline
methylen blue &normal saline DYE
Washing after dye injection
methylene blue
metheylne blue with normal saline
(Active Comparator)
20 ml methylene blue injected without washing
methylene blue

Primary Outcomes

Pregnancy rate
time frame: up to 12 weeks

Secondary Outcomes

complications rate in two group
time frame: Up to 12 weeks

Eligibility Criteria

Female participants from 20 years up to 38 years old.

Inclusion Criteria: - women with unexplained infertility Exclusion Criteria: - any intervention during operation like ovarian drilling or septum resection

Additional Information

Description women with unexplained infertility and without any problem during laparoscopy and hysteroscopy entered in the study.After randomization in one group after dye test,washing with normal saline was done until all of visible Methylene blue removed.In second group washing with normal saline did not done. pregnancy rate until three months after operation was followed without any intervention.
Trial information was received from ClinicalTrials.gov and was last updated in November 2012.
Information provided to ClinicalTrials.gov by Pourmatroud, Elham, M.D..