Overview

This trial is active, not recruiting.

Condition male infertility
Treatments biospecimen staining
Sponsor Hillel Yaffe Medical Center
Start date January 2010
End date May 2010
Trial size 25 participants
Trial identifier NCT01178463, HYMC-0065-09

Summary

The researchers hypothesized that nonobstructive azoospermia might be associated with a reduction OF SSCs in seminiferous tubules as compared with obstructive azoospermia. Testicular sperm specimens that had been previously extracted from azoospermic patients will be paraffin embedded and stained with anti GPR-125. After antigen retrieval, the sections will be incubated with rabbit anti GPR125 followed by Cy3-conjugated anti rabbit secondary antibodies. Those found to be SSCs will be counted using light microscopy, and compared between patients with obstructive (n=11) and non-obstructive azoospermia (n=9).

United States No locations recruiting
Other Countries No locations recruiting

Study Design

Observational model cohort
Time perspective retrospective
Arm
biospecimen staining
The sections will be incubated with rabbit anti GPR125 followed by Cy3-conjugated anti rabbit secondary antibodies.
biospecimen staining
The sections will be incubated with rabbit anti GPR125 followed by Cy3-conjugated anti rabbit secondary antibodies.

Primary Outcomes

Measure
SSCs were identified in both obstructive and nonobstructive azoospermia patients.
time frame: One year

Eligibility Criteria

Male participants at least 18 years old.

Inclusion Criteria: - Azoospermic patients Exclusion Criteria: - Patients with sperm cells

Trial information was received from ClinicalTrials.gov and was last updated in August 2010.
Information provided to ClinicalTrials.gov by Hillel Yaffe Medical Center.