Spermatogonial Stem Cells in Azoospermic Patients: a Comparison Between Obstructive and Non-obstructive Azoospermia
This trial is active, not recruiting.
|Sponsor||Hillel Yaffe Medical Center|
|Start date||January 2010|
|End date||May 2010|
|Trial size||25 participants|
|Trial identifier||NCT01178463, HYMC-0065-09|
The researchers hypothesized that nonobstructive azoospermia might be associated with a reduction OF SSCs in seminiferous tubules as compared with obstructive azoospermia. Testicular sperm specimens that had been previously extracted from azoospermic patients will be paraffin embedded and stained with anti GPR-125. After antigen retrieval, the sections will be incubated with rabbit anti GPR125 followed by Cy3-conjugated anti rabbit secondary antibodies. Those found to be SSCs will be counted using light microscopy, and compared between patients with obstructive (n=11) and non-obstructive azoospermia (n=9).
SSCs were identified in both obstructive and nonobstructive azoospermia patients.
time frame: One year
Male participants at least 18 years old.
Inclusion Criteria: - Azoospermic patients Exclusion Criteria: - Patients with sperm cells
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