Study in an Ex-vivo Thrombosis Model With Human Umbilical Vein Endothelial Cells (HUVEC)-Perfusion
This trial is active, not recruiting.
|Conditions||thrombosis, thrombus formation, thrombus stability|
|Sponsor||Medical University of Vienna|
|Start date||November 2009|
|End date||September 2011|
|Trial size||30 participants|
|Trial identifier||NCT01020110, HUVEC-Version1|
Evaluation of fibrin and platelet deposition on a human umbilical endothelial cell surface in perfusion chamber experiments using human whole blood.
Open-label, non interventional study Perfusion chamber experiment will be performed in 30 healthy patients. The Impact of different pH-solutions on thrombus lysis will be evaluated in an in-vitro second step
D-Dimer content of the thrombus reflecting the size of the thrombus.
|Observational model||case control|
D-Dimer content of the plasmin degraded thrombus
time frame: Assessment will follow after all perfusion chamber experiments (1 week)
Male or female participants at least 18 years old.
Inclusion Criteria: - Age > 18 years - Written informed consent Exclusion Criteria: - Use of any medication - Current diseases - Anemia
|Official title||Human Umbilical Vein Endothelial Cells (HUVEC)-Perfusion|
|Principal investigator||Michael Wolzt, MD|
|Description||All Subjects will have two perfusion chamber experiment as follow with an estimated maximum of blood loss of 165ml: Before blood perfusion, perfuse the system including the chambers with human umbilical vein endothelial cell coated glass slides (HUVEC), with sodium chloride (NaCl) (0.9%), to ensure no leaks and to remove all air bubbles. Blood is drawn from a vein in the arm with a Surflo® Winged Infusion Set, 19G (Terumo Europe, Leuven, Belgium) with a pump (Masterflex® L/S™, Cole-Parmer Instrument Company, Vernon Hills, Illinois, USA). Five mL of blood is discarded before each perfusion. Three in serial placed flow chambers (with HUVEC) heated to 37°C are used. They are made of a Plexiglas block through which a cylindrical hole of 0.2 cm in diameter is machined. The aorta pieces are perfused at 10 mL/min for 5 minutes, followed by a 30 seconds perfusion with NaCl (0.9%). The plasmin degraded thrombus D-Dimer content will be further evaluated under different conditions (different pH solutions).|
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