This trial is active, not recruiting.

Condition malaria
Sponsor University of Minnesota - Clinical and Translational Science Institute
Start date March 2008
End date March 2019
Trial size 20 participants
Trial identifier NCT00850655, 0607M88827


This is a study of the genetics of malaria transmission by the mosquito vector in Africa, Anopheles gambiae. The study focuses on the mosquito vector, not the human subjects infected with malaria. DNA extracted from infected mosquitoes will be analyzed genetically by microsatellite and single-nucleotide polymorphisms (SNPs) to identify mosquito genes that control mosquito resistance to malaria parasites.

United States No locations recruiting
Other countries No locations recruiting

Study Design

Observational model ecologic or community
Time perspective cross-sectional

Primary Outcomes

mosquito infection
time frame: one week

Eligibility Criteria

Male or female participants from 5 years up to 10 years old.

Inclusion Criteria: - 5-10 years old, and general good health Exclusion Criteria: - Outside age group - Specific diagnosable health problems - General poor health

Additional Information

Official title Genetic Association Mapping of Malaria Resistance in Anopheles Gambiae
Principal investigator Kenneth D Vernick, PhD
Description A standard diagnostic blood smear from a drop of blood will be carried out on 20 study subjects once per month. One malaria-positive subject from among the 20 will be asked to donate 5cc of blood, also no more frequently than once per month. The blood will be placed in a water-jacketed heated membrane feeding device that will be positioned on top of a cage of mosquitoes. The mosquitoes are attracted to the warmth and drink the blood through the artificial skin-like membrane. The goal of the research is to study the infected mosquitoes. No studies will be carried out on the human subjects. The point of human subjects involvement is to obtain the naturally malaria-infected blood for feeding of mosquitoes. After blood feeding, the mosquitoes are held in an environmental chamber for one week, at which time they are dissected to count numbers of malaria parasites developing on the mosquito stomach wall. This number constitutes the quantitative phenotype, and we extract genomic DNA from the carcass for genetic marker typing, which constitutes the genotype. Genetic linkage analysis identifies markers near genes that control parasite number, thus resistance or susceptibility to parasite infection.
Trial information was received from ClinicalTrials.gov and was last updated in March 2014.
Information provided to ClinicalTrials.gov by University of Minnesota - Clinical and Translational Science Institute.